Platelet storage at cold temperature (CT, 2–6 ☌), which was performed until the 1980s, offers an alternative to reduce the risk of bacterial growth and to prolong the storage period 4, 5, 6. Therefore, according to manufacturing conditions and legal regulations, the shelf life of PC is commonly restricted to 4–7 days in many countries. Currently, PC are regularly stored at room temperature (RT, 20–24 ☌), accompanied by two major disadvantages: the risk of bacterial growth and the development of functional restrictions called storage lesions 2, 3. In transfusion medicine, platelet concentrates (PC) are frequently manufactured blood components, particularly used for prophylaxis or treatment of hemorrhage due to thrombocytopenia or for patients with reduced platelet function 1.
Short-term refrigeration may be considered as a rational approach to obtain PC with higher functional reactivity for the treatment of hemorrhage.
In conclusion, a time period of 1–2 h for refrigeration is sufficient to induce an attenuation of inhibitory signaling, accompanied with an enhancement of platelet responsiveness.
Furthermore, platelet-covered areas on collagen-coated slides and aggregate formation under flow conditions were increased after storage at CT, in addition to induced activation markers. Concomitantly, ADP- and collagen-induced threshold aggregation values were enhanced by up to 30–40%. Compared to RT, basal and induced vasodilator-stimulated phosphoprotein (VASP) phosphorylation levels were decreased under CT within 1 h by approximately 20%, determined by Western blot analysis and flow cytometry. Thus, freshly prepared platelet-rich-plasma (PRP) was stored for 1 and 2 h at CT (2–6 ☌) or at RT (20–24 ☌), followed by subsequent comparative analysis.
For developing strategies in blood banking, it is required to elucidate the time-dependent onset of facilitated platelet activation. Recently, we could show that cold-induced attenuation of inhibitory signaling is an important mechanism promoting platelet reactivity. Storage of platelet concentrates (PC) at cold temperature (CT) is discussed as an alternative to the current standard of storage at room temperature (RT).
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